Simple Apparatus for One-Dimensional Ascending Paper Chromatography

A Simple Apparatus for Thin-layer Chromatography.

Thin-layer chromatography was introduced by Stahl (1956) and has advantages over paper chromatography in speed, resolution, and smaller sample size. Also, the physical nature of the thin layer allows its treatment, where necessary, with strong acids and alkalis. It has been found possible to construct a satisfactory, cheap, and simple apparatus for the preparation of thin layers on glass carrie...

A Simple, Rapid Method for Paper Chromatography of Barbiturates.

Barbiturates are extracted quantitatively into ethyl ether from biologic fluids buffered to pH 6. A measured portion of the ether extract is then impinged to dryness and the residue spotted on Whatman No. 2 paper that has been previously dipped in alkali and dried. The paper is developed by descending chromatography with chloroform saturated with water. The barbiturate may then be located and a...

The quantitative determination of amino-acids in urine used in conjunction with one-dimensional paper chromatography.

The value of paper chromatography in the study of the amino-acids of urine in disease has been shown in particular by Dent (1946, 1948). Although present methods of oneor two-dimensional piper chromatography can indicate important quantitative changes to the observer, there is some difficulty in conveying impressions to another, e.g., to a clinician or to a reader of a paper. It does appear tha...

Automated tank for one- or two-dimensional thin-layer chromatography.

An automated one- or two-dimensional TLC tank is described. The solvent front is monitored by a photocell or recycling timers.

Study of phenylketonuria by paper chromatography